Gene expression differences in infected and noninfected middle ear complementary DNA libraries.

ABSTRACT:

Objectives

To investigate genetic differences in middle ear mucosa (MEM) with nontypeable Haemophilus influenzae (NTHi) infection. Genetic upregulation and downregulation occurs in MEM during otitis media (OM) pathogenesis. A comprehensive assessment of these genetic differences using the techniques of complementary DNA (cDNA) library creation has not been performed.

Design

The cDNA libraries were constructed from NTHi-infected and noninfected chinchilla MEM. Random clones were picked, sequenced bidirectionally, and submitted to the National Center for Biotechnology Information (NCBI) Expressed Sequence Tags database, where they were assigned accession numbers. These numbers were used with the basic local alignment search tool (BLAST) to align clones against the nonredundant

SUBMITTER: Kerschner JE 

PROVIDER: S-EPMC2912141 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature