Transcriptional profiles of leukocyte populations provide a tool for interpreting gene expression patterns associated with high fat diet in mice.

ABSTRACT:

Background

Microarray experiments in mice have shown that high fat diet can lead to elevated expression of genes that are disproportionately associated with immune functions. These effects of high fat (atherogenic) diet may be due to infiltration of tissues by leukocytes in coordination with inflammatory processes.

Methodology/principal findings

The Novartis strain-diet-sex microarray database (GSE10493) was used to evaluate the hepatic effects of high fat diet (4 weeks) in 12 mouse strains and both genders. We develop and apply an algorithm that identifies "signature transcripts" for many different leukocyte populations (e.g., T cells, B cells, macrophages) and uses this information to derive an in silico "inflammation profile". Inflammation profiles highlighted monocytes,

SUBMITTER: Swindell WR 

PROVIDER: S-EPMC2912331 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature