Rad51 inhibits translocation formation by non-conservative homologous recombination in Saccharomyces cerevisiae.

ABSTRACT: Chromosomal translocations are a primary biological response to ionizing radiation (IR) exposure, and are likely to result from the inappropriate repair of the DNA double-strand breaks (DSBs) that are created. An abundance of repetitive sequences in eukaryotic genomes provides ample opportunity for such breaks to be repaired by homologous recombination (HR) between non-allelic repeats. Interestingly, in the budding yeast, Saccharomyces cerevisiae the central strand exchange protein, Rad51 that is required for DSB repair by gene conversion between unlinked repeats that conserves genomic structure also suppresses translocation formation by several HR mechanisms. In particular, Rad51 suppresses translocation formation by single-strand annealing (SSA), perhaps the most efficient mechanism for

SUBMITTER: Manthey GM 

PROVIDER: S-EPMC2912366 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature