Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins.

ABSTRACT: UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreG(Str)) and selected site-directed variants of UreG(Str) were constructed for studying the in vivo effects on urease activation in recombinant Escherichia coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro protein-protein interactions. Whereas the Strep tag had no effect on UreG's ability to activate urease, enzyme activity was essentially abolished in the K20A, D49A, C72A, H74A, D80A, and S111A UreG(Str) variants, with diminished activity also noted with E25A, C28A, and S115A proteins. Lys20 and Asp49 are likely to function in binding/hydrolysis of GTP and binding of Mg, respectively. UreG(Str) binds o

SUBMITTER: Boer JL 

PROVIDER: S-EPMC2912431 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature