Characterization of Diaphanous-related formin FMNL2 in human tissues.

ABSTRACT:

Background

Diaphanous-related formins govern actin-based processes involved in many cellular functions, such as cell movement and invasion. Possible connections to developmental processes and cellular changes associated with malignant phenotype make them interesting study targets. In spite of this, very little is known of the tissue distribution and cellular location of any mammalian formin. Here we have carried out a comprehensive analysis of the formin family member formin -like 2 (FMNL2) in human tissues.

Results

An FMNL2 antibody was raised and characterized. The affinity-purified FMNL2 antibody was validated by Western blotting, Northern blotting, a peptide competition assay and siRNA experiments. Bioinformatics-based mRNA profiling indicated that FMNL2 is widely expres

SUBMITTER: Gardberg M 

PROVIDER: S-EPMC2912821 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature