Different integration site structures between L1 protein-mediated retrotransposition in cis and retrotransposition in trans.

ABSTRACT:

Background

Long interspersed nuclear element-1 (LINE-1 or L1) is a dominant repetitive sequence in the human genome. Besides mediating its own retrotransposition, L1 can mobilize Alu and messenger RNA (mRNA) in trans, and probably also SVA and non-coding RNA. The structures of L1 copies and trans-mobilized retrocopies are variable and can be classified into three categories: full-length; 5'-truncated; and 5'-inverted insertions. These structures may be generated by different 5' integration mechanisms.

Results

In this study, a method to correctly characterize insertions with short target site duplications (TSDs) is developed and extranucleotides, TSDs and microhomologies (MHs) at junctions were analysed for the three types of insertions. Only 5'-truncated L1 insertions were f

SUBMITTER: Kojima KK 

PROVIDER: S-EPMC2912911 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature