The nature of peptides presented by an HLA class I low expression allele.

ABSTRACT:

Background

The functional integrity of human leukocyte antigen low expression variants is a prerequisite for considering them as essential in the matching process of hematopoietic stem cell donors and recipients to diminish the risk of serious complications such as graft-versus-host disease or graft rejection. The HLA-A*3014L variant has a disulfide bridge missing in the alpha2 domain which could affect peptide binding and presentation to T cells.

Design and methods

HLA-A*3014L and HLA-A*3001 were expressed as truncated variants and peptides were eluted and subjected to pool sequencing by Edman degradation as well as to single-peptide sequencing by mass spectrometry. Quantitative analysis of binding peptides presented in vivo was performed by a flow cytometric peptide-bindin

SUBMITTER: Hinrichs J 

PROVIDER: S-EPMC2913087 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature