TRB3 function in cardiac endoplasmic reticulum stress.

ABSTRACT:

Rationale

Tribbles (TRB)3 is an intracellular pseudokinase that modulates the activity of several signal transduction cascades. TRB3 has been reported to inhibit the activity of Akt protein kinases. TRB3 gene expression is highly regulated in many cell types, and amino acid starvation, hypoxia, or endoplasmic reticulum (ER) stress promotes TRB3 expression in noncardiac cells.

Objective

The objective of this work was to examine TRB3 expression and function in cultured cardiac myocytes and in mouse heart.

Methods and results

Agents that induced ER stress increased TRB3 expression in cultured cardiac myocytes while blocking insulin-stimulated Akt activation in these cells. Knockdown of TRB3 in cultured cardiac myocytes reversed the effects of ER stress on insulin signali

SUBMITTER: Avery J 

PROVIDER: S-EPMC2913227 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature