Transport mechanisms of carnosine in SKPT cells: contribution of apical and basolateral membrane transporters.

ABSTRACT:

Purpose

The aim of this study was to investigate the transport properties of carnosine in kidney using SKPT cell cultures as a model of proximal tubular transport, and to isolate the functional activities of renal apical and basolateral transporters in this process.

Methods

The membrane transport kinetics of 10 microM [3H]carnosine was studied in SKPT cells as a function of time, pH, potential inhibitors and substrate concentration. A cellular compartment model was constructed in which the influx, efflux and transepithelial clearances of carnosine were determined. Peptide transporter expression was probed by RT-PCR.

Results

Carnosine uptake was 15-fold greater from the apical than basolateral surface of SKPT cells. However, the apical-to-basolateral transepithelial tr

SUBMITTER: Jappar D 

PROVIDER: S-EPMC2913304 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature