Study of cytotoxic and therapeutic effects of stable and purified silver nanoparticles on tumor cells.

ABSTRACT: We have synthesized and purified silver nanoparticles (Ag NPs) (11.3+/-2.3 nm) that are stable (non-aggregated) in cell culture medium and inside single living cells. We have developed new imaging methods to characterize sizes and number of single NPs in the medium and in single living cells in real-time and determine their stability (non-aggregation) in the medium and in single living cells at single NP resolution. These new approaches allow us to study toxic and therapeutic effects of single Ag NPs on tumor cells (L929, mouse fibroblast cells) with determined sizes and concentrations (doses) of NPs over time at single NP and single cell resolution. We found that Ag NPs inhibited the growth and division of tumor cells and their nuclei, in a dose and time dependent manner, showing signific

SUBMITTER: Nallathamby PD 

PROVIDER: S-EPMC2914687 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature