Molecular identification of adenovirus causing respiratory tract infection in pediatric patients at the University of Malaya Medical Center.

ABSTRACT:

Background

There are at least 51 adenovirus serotypes (AdV) known to cause human infections. The prevalence of the different human AdV (HAdV) serotypes varies among different regions. Presently, there are no reports of the prevalent HAdV types found in Malaysia. The present study was undertaken to identify the HAdV types associated primarily with respiratory tract infections (RTI) of young children in Malaysia.

Methods

Archived HAdV isolates from pediatric patients with RTI seen at the University of Malaya Medical Center (UMMC), Kuala Lumpur, Malaysia from 1999 to 2005 were used. Virus isolates were inoculated into cell culture and DNA was extracted when cells showed significant cytopathic effects. AdV partial hexon gene was amplified and the sequences together with other kn

SUBMITTER: Abd-Jamil J 

PROVIDER: S-EPMC2914739 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature