Proteomic analysis reveals molecular biological details in varioliform gastritis without Helicobacter pylori infection.

ABSTRACT:

Aim

To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection, prevention and intervention of gastric cancer.

Methods

A combination of two-dimensional gel electrophoresis and mass spectrometry was used to detect the differentially expressed proteins between varioliform gastritis and matched normal mucosa. The selected proteins were confirmed by Western blotting and reverse transcription polymerase chain reaction (RT-PCR) in additional samples and the function of some proteins in varioliform gastritis was analyzed by bio-method preliminarily.

Results

We identified 21 differentially expressed proteins in varioliform gastritis, and compared them with matched normal mucosa. Eleven proteins were upregulated and ten downregula

SUBMITTER: Zhang L 

PROVIDER: S-EPMC2915426 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature