Particle tracking model of electrophoretic morphogen movement reveals stochastic dynamics of embryonic gradient.

ABSTRACT: Some developmental events rely on an electrophoretic force to produce morphogenetic gradients. To quantitatively explore the dynamics of this process, we constructed a stochastic model of an early phase of left-right patterning: serotonin movement through the gap junction-coupled blastomeres of the Xenopus embryo. Particle-tracking simulations showed that a left-right gradient is formed rapidly, quickly reaching a final stable level. The voltage difference was critical for producing a morphogen gradient of the right steepness; gap junctional connectivity and morphogen mass determined the timing of the gradient. Endogenous electrophoresis drives approximately 50% of the particles across more than one cell width, and approximately 20% can travel across half the embryo. The stochastic behavio

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC2915568 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature