Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection.

ABSTRACT:

Background

Viral and bacterial diseases can cause mass mortalities in commercial shrimp aquaculture. In contrast to studies on the antiviral response, the responses of shrimps to bacterial infections by high throughput techniques have been reported only at the transcriptional level and not at the translational level. In this study, a proteomic analysis of shrimp hemocytes to identify differentially expressed proteins in response to a luminous bacterium Vibrio harveyi was evaluated for its feasibility and is reported for the first time.

Results

The two-dimensional gel electrophoresis (2-DE) patterns of the hemocyte proteins from the unchallenged and V. harveyi challenged shrimp, Penaeus monodon, at 24 and 48 h post infection were compared. From this, 27 differentially express

SUBMITTER: Somboonwiwat K 

PROVIDER: S-EPMC2915975 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature