Complementation of Listeria monocytogenes null mutants with selected Listeria seeligeri virulence genes suggests functional adaptation of Hly and PrfA and considerable diversification of prfA regulation in L. seeligeri.

ABSTRACT: While Listeria seeligeri and L. monocytogenes contain the main Listeria virulence gene cluster, only L. monocytogenes is considered an intracellular pathogen. Initial evolutionary analyses showed that the virulence genes prfA, hly, and plcA are conserved in L. seeligeri, with specific Hly and PrfA amino acid residues showing evidence for positive selection in L. seeligeri. Our data also show that temperature-dependent transcript patterns for prfA, which encodes a transcriptional regulator of virulence genes, differed between L. monocytogenes and L. seeligeri. To further investigate the divergence of virulence gene function and regulation, L. seeligeri prfA (prfA(LS)), hly (hly(LS)), and plcA (plcA(LS)), as well as prfA(LS) constructs with different prfA promoter regions, were introduced in

SUBMITTER: Stelling CR 

PROVIDER: S-EPMC2916458 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature