Improved dsrA-based terminal restriction fragment length polymorphism analysis of sulfate-reducing bacteria.

ABSTRACT: To better describe the community structure of sulfate-reducing bacteria in environmental systems, we compared several dissimilatory sulfite reductase (dsr) primer sets for terminal restriction fragment length polymorphism application. A new reverse primer that increased allelic diversity estimates up to 5-fold was applied to hydrocarbon seep samples to examine the relationship between guild activity and diversity.

SUBMITTER: Santillano D 

PROVIDER: S-EPMC2916486 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature