Requirement of purine and pyrimidine synthesis for colonization of the mouse intestine by Escherichia coli.

ABSTRACT: To study the adaptation of an intestinal bacterium to its natural environment, germfree mice were associated with commensal Escherichia coli MG1655. Two-dimensional gel electrophoresis was used to identify proteins differentially expressed in E. coli MG1655 collected from either cecal contents or anaerobic in vitro cultures. Fourteen differentially expressed proteins (>3-fold; P < 0.05) were identified, nine of which were upregulated in cecal versus in vitro-grown E. coli. Four of these proteins were investigated further for their role in gut colonization. After deletion of the corresponding genes, the resulting E. coli mutants were tested for their ability to colonize the intestines of gnotobiotic mice in competition with the wild-type strain. A mutant devoid of ydjG, which encodes a puta

SUBMITTER: Vogel-Scheel J 

PROVIDER: S-EPMC2916491 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature