Uptake, distribution and diffusivity of reactive fluorophores in cells: implications toward target identification.

ABSTRACT: There is much recent interest in the application of copper-free click chemistry to study a wide range of biological events in vivo and in vitro. Specifically, azide-conjugated fluorescent probes can be used to identify targets which have been modified with bioorthogonal reactive groups. For intracellular applications of this chemistry, the structural and physicochemical properties of the fluorescent azide become increasingly important. Ideal fluorophores should extensively accumulate within cells, have even intracellular distribution, and be free (unbound), allowing them to efficiently participate in bimolecular reactions. We report here on the synthesis and evaluation of a set of structurally diverse fluorescent probes to examine their potential usefulness in intracellular click reactions

SUBMITTER: Cunningham CW 

PROVIDER: S-EPMC2916926 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature