Imaging beta-galactosidase activity in human tumor xenografts and transgenic mice using a chemiluminescent substrate.

ABSTRACT:

Background

Detection of enzyme activity or transgene expression offers potential insight into developmental biology, disease progression, and potentially personalized medicine. Historically, the lacZ gene encoding the enzyme beta-galactosidase has been the most common reporter gene and many chromogenic and fluorogenic substrates are well established, but limited to histology or in vitro assays. We now present a novel approach for in vivo detection of beta-galactosidase using optical imaging to detect light emission following administration of the chemiluminescent 1,2-dioxetane substrate Galacto-Light PlusTM.

Methodology and principal findings

B-gal activity was visualized in stably transfected human MCF7-lacZ tumors growing in mice. LacZ tumors were identified versus contral

SUBMITTER: Liu L 

PROVIDER: S-EPMC2917367 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature