Stem cell proliferation during in vitro development of the model cestode Mesocestoides corti from larva to adult worm.

ABSTRACT:

Background

In free-living flatworms somatic differentiated cells do not divide, and a separate population of stem cells (called neoblasts) is responsible for cell proliferation and renewal. In cestodes, there is evidence that similar mechanisms of cell renewal exist.

Results

In this work, we have characterized proliferative cells during the development of the model cestode Mesocestoides corti from larva (tetrathyridium) to young segmented worm. This was done by two complementary strategies with congruent results: characterizing cells in S phase and their progeny by incorporation of 5-bromo-2'-deoxyuridine, and characterizing cells in M phase by arresting mitotic cells with colchicine and studying their morphology and distribution. Proliferative cells are localized only in th

SUBMITTER: Koziol U 

PROVIDER: S-EPMC2917415 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature