Hematopoietic and nonhematopoietic cell tissue factor activates the coagulation cascade in endotoxemic mice.

ABSTRACT: Tissue factor (TF) is the primary activator of the coagulation cascade. During endotoxemia, TF expression leads to disseminated intravascular coagulation. However, the relative contribution of TF expression by different cell types to the activation of coagulation has not been defined. In this study, we investigated the effect of either a selective inhibition of TF expression or cell type-specific deletion of the TF gene (F3) on activation of coagulation in a mouse model of endotoxemia. We found that inhibition of TF on either hematopoietic or nonhematopoietic cells reduced plasma thrombin-antithrombin (TAT) levels 8 hours after administration of bacterial lipopolysaccharide (LPS). In addition, plasma TAT levels were significantly reduced in endotoxemic mice lacking the TF gene in either my

SUBMITTER: Pawlinski R 

PROVIDER: S-EPMC2918334 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature