High-throughput analysis of total plasma fatty acid composition with direct in situ transesterification.

ABSTRACT:

Background

Plasma fatty acid (FA) composition reflects dietary intake and endogenous turnover and is associated with health outcomes on a short and long term basis. The total plasma FA pool represents the composition of all FA containing lipid fractions. We developed a simplified and affordable high-throughput method for the analysis of total plasma FA composition, suitable for large studies.

Methodology/principal findings

The total lipid FA from 100 microl plasma is transferred in situ into methyl esters, avoiding initial extraction and drying steps. The fatty acid methyl esters are extracted once and analyzed by gas chromatography. For the new direct in situ transesterification method optimal, reaction parameters were determined. Intra-assay analysis (n=8) revealed coeffic

SUBMITTER: Glaser C 

PROVIDER: S-EPMC2918509 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature