An efficient strategy to induce and maintain in vitro human T cells specific for autologous non-small cell lung carcinoma.

ABSTRACT:

Background

The efficient expansion in vitro of cytolytic CD8+ T cells (CTLs) specific for autologous tumors is crucial both for basic and translational aspects of tumor immunology. We investigated strategies to generate CTLs specific for autologous Non-Small Cell Lung Carcinoma (NSCLC), the most frequent tumor in mankind, using circulating lymphocytes.

Principal findings

Classic Mixed Lymphocyte Tumor Cultures with NSCLC cells consistently failed to induce tumor-specific CTLs. Cross-presentation in vitro of irradiated NSCLC cells by autologous dendritic cells, by contrast, induced specific CTL lines from which we obtained a high number of tumor-specific T cell clones (TCCs). The TCCs displayed a limited TCR diversity, suggesting an origin from few tumor-specific T cell precu

SUBMITTER: Canderan G 

PROVIDER: S-EPMC2918513 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature