Project description:Gene expression during stationary phase and symbiosis of R. etli CFN42 was compared to that of exponentially growing cells. This allowed us to better understand how R. etli adapts to a non-growing lifestyle, both the free-living and symbiotic state, as well as to determine to what extent this adaptation is similar in both states. R. etli CFN42 was grown at 30˚C in AMS medium supplied with 10 mM NH4Cl and 10 mM succinate while monitoring the optical density (OD) of the culture. Free-living samples were taken at OD600 = 0.3 and 6 hours after reaching the maximum OD, representing early exponential and stationary phase respectively. Bacteroid samples were obtained from nodules 3 weeks after inoculation of Common bean plants (Phaseolus vulgaris cv Limburgse vroege).

2010-11-04 | E-GEOD-25094 | biostudies-arrayexpress

Structures and Anti-Allergic Activities of Natural Products from Marine Organisms.

Project description: Not available

| S-EPMC10056057 | biostudies-literature

Comparison of transcriptome between free-living and symbiotic cells of Frankia alni ACN14a

Project description:The actinobacteria Frankia alni is able to induce the formation of nodules on the root of a large spectrum of actinorhizal plants, where it converts dinitrogen to ammonia in exchange for plant photosynthates. In the present study, transcriptional analyses were performed on nitrogen-replete free-living cells and on Alnus glutinosa nodule bacteria, using whole genome microarrays. Distribution of nodule-induced genes on the genome was found to be mostly over regions with high synteny between three Frankia genomes, while nodule-repressed genes, which were mostly hypothetical and not conserved, were spread around the genome. Genes known to be related to symbiosis were highly induced: nif (nitrogenase), hup2 (hydrogenase uptake), suf (sulfur-iron cluster) and shc (hopanoids synthesis). The expression of genes involved in ammonium assimilation and transport was strongly modified suggesting that bacteria ammonium assimilation was limited. Genes involved in particular in transcriptional regulation, signalling processes, protein drug export, protein secretion, lipopolysaccharide and peptidoglycan biosynthesis that may play a role in symbiosis were also identified. We showed that this nodule transcriptome of Frankia was highly similar among phylogenetically distant plant families.

2010-09-12 | GSE18190 | GEO

Comparison of transcriptome between free-living and symbiotic cells of Frankia alni ACN14a

2010-09-12 | E-GEOD-18190 | biostudies-arrayexpress

Metagenomic Approaches to Identify Novel Organisms from the Soil Environment in a Classroom Setting.

Project description: Not available

| S-EPMC5134946 | biostudies-literature

S. meliloti 1021 WT and ΔcspA2 ΔcspA4 whole transcriptome sequencing from free-living and symbiotic nodules

Project description:Rhizobia are soil bacteria that can associate with some legumes and participate in symbiotic nitrogen fixation. Bacterial CspA family members are small, single stranded nucleic acid binding proteins. Differentiation of rhizobial bacteria from a free-living to symbiotic state within legume root nodules follows a massive re-programming of bacterial gene expression. Here, the role of Sinorhizobium meliloti CspA family members in symbiotic development with Medicago sativa (alfalfa) was investigated. We defined expression patterns of CspA family members, identified CspA interacting RNAs, and investigated phenotypes and transcriptional defects associated with cspA deletion strains. We propose that these proteins affect rhizobial physiology through their global control of the cellular RNA secondary structure strength environment and through specific modulation of small non-coding RNA (sRNA) structures involved in cis-regulation of stress responsive sigma factor expression. This work describes an RNA structure mediated mechanism important for bacterial stress adaptation and symbiotic development within a plant host.

2018-08-01 | GSE102800 | GEO

Specific hopanoid classes differentially affect free-living and symbiotic states of Bradyrhizobium diazoefficiens.

Project description: Not available

| S-EPMC4620461 | biostudies-literature

Diverse roles of the GlcP glucose permease in free-living and symbiotic cyanobacteria.

Project description: Not available

| S-EPMC4091242 | biostudies-literature