Blood-brain barrier breakdown and repair by Src after thrombin-induced injury.

ABSTRACT:

Objective

Thrombin mediates the life-threatening cerebral edema that occurs after intracerebral hemorrhage. Therefore, we examined the mechanisms of thrombin-induced injury to the blood-brain barrier (BBB) and subsequent mechanisms of BBB repair.

Methods

Intracerebroventricular injection of thrombin (20U) was used to model intraventricular hemorrhage in adult rats.

Results

Thrombin reduced brain microvascular endothelial cell (BMVEC) and perivascular astrocyte immunoreactivity-indicating either cell injury or death-and functionally disrupted the BBB as measured by increased water content and extravasation of sodium fluorescein and Evans blue dyes 24 hours later. Administration of nonspecific Src family kinase inhibitor (PP2) immediately after thrombin injections block

SUBMITTER: Liu DZ 

PROVIDER: S-EPMC2919346 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature