Regulation of early signaling and gene expression in the alpha-particle and bystander response of IMR-90 human fibroblasts.

ABSTRACT:

Background

The existence of a radiation bystander effect, in which non-irradiated cells respond to signals from irradiated cells, is well established. To understand early signaling and gene regulation in bystander cells, we used a bio-informatics approach, measuring global gene expression at 30 minutes and signaling pathways between 30 minutes and 4 hours after exposure to alpha-particles in IMR-90 fibroblasts.

Methods

We used whole human genome microarrays and real time quantitative PCR to measure and validate gene expression. Microarray analysis was done using BRB-Array Tools; pathway and ontology analyses were done using Ingenuity Pathway Analysis and PANTHER, respectively. We studied signaling in irradiated and bystander cells using immunoblotting and semi-quantitative i

SUBMITTER: Ghandhi SA 

PROVIDER: S-EPMC2919438 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature