A novel cell-free mitochondrial fusion assay amenable for high-throughput screenings of fusion modulators.

ABSTRACT:

Background

Mitochondria are highly dynamic organelles whose morphology and position within the cell is tightly coupled to metabolic function. There is a limited list of essential proteins that regulate mitochondrial morphology and the mechanisms that govern mitochondrial dynamics are poorly understood. However, recent evidence indicates that the core machinery that governs mitochondrial dynamics is linked within complex intracellular signalling cascades, including apoptotic pathways, cell cycle transitions and nuclear factor kappa B activation. Given the emerging importance of mitochondrial plasticity in cell signalling pathways and metabolism, it is essential that we develop tools to quantitatively analyse the processes of fission and fusion. In terms of mitochondrial fusion, the

SUBMITTER: Schauss AC 

PROVIDER: S-EPMC2919466 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature