Serious limitations of the QTL/microarray approach for QTL gene discovery.

ABSTRACT:

Background

It has been proposed that the use of gene expression microarrays in nonrecombinant parental or congenic strains can accelerate the process of isolating individual genes underlying quantitative trait loci (QTL). However, the effectiveness of this approach has not been assessed.

Results

Thirty-seven studies that have implemented the QTL/microarray approach in rodents were reviewed. About 30% of studies showed enrichment for QTL candidates, mostly in comparisons between congenic and background strains. Three studies led to the identification of an underlying QTL gene. To complement the literature results, a microarray experiment was performed using three mouse congenic strains isolating the effects of at least 25 biometric QTL. Results show that genes in the congenic

SUBMITTER: Verdugo RA 

PROVIDER: S-EPMC2919467 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature