High prevalence of antibodies against polyomavirus WU, polyomavirus KI, and human bocavirus in German blood donors.

ABSTRACT:

Background

DNA of the polyomaviruses WU (WUPyV) and KI (KIPyV) and of human bocavirus (HBoV) has been detected with varying frequency in respiratory tract samples of children. However, only little is known about the humoral immune response against these viruses. Our aim was to establish virus-specific serological assays and to determine the prevalence of immunoglobulin G (IgG) against these three viruses in the general population.

Methods

The capsid proteins VP1 of WUPyV and KIPyV and VP2 of HBoV were cloned into baculovirus vectors and expressed in Sf9 insect cells. IgG antibodies against WUPyV VP1, KIPyV VP1, and HBoV VP2 were determined by immunofluorescence assays in 100 plasma samples of blood donors.

Results

The median age of the blood donors was 31 years (range

SUBMITTER: Neske F 

PROVIDER: S-EPMC2919546 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature