Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq.

ABSTRACT: Both transcription and post-transcriptional processes, such as alternative splicing, play crucial roles in controlling developmental programs in metazoans. Recently emerged RNA-seq method has brought our understanding of eukaryotic transcriptomes to a new level, because it can resolve both gene expression level and alternative splicing events simultaneously. To gain a better understanding of cellular differentiation in gonads, we analyzed mRNA profiles from Drosophila testes and ovaries using RNA-seq. We identified a set of genes that have sex-specific isoforms in wild-type (WT) gonads, including several transcription factors. We found that differentiation of sperms from undifferentiated germ cells induced a dramatic downregulation of RNA splicing factors. Our data confirmed that RNA splic

SUBMITTER: Gan Q 

PROVIDER: S-EPMC2919574 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature