Large-scale detection and analysis of RNA editing in grape mtDNA by RNA deep-sequencing.

ABSTRACT: RNA editing is a widespread post-transcriptional molecular phenomenon that can increase proteomic diversity, by modifying the sequence of completely or partially non-functional primary transcripts, through a variety of mechanistically and evolutionarily unrelated pathways. Editing by base substitution has been investigated in both animals and plants. However, conventional strategies based on directed Sanger sequencing are time-consuming and effectively preclude genome wide identification of RNA editing and assessment of partial and tissue-specific editing sites. In contrast, the high-throughput RNA-Seq approach allows the generation of a comprehensive landscape of RNA editing at the genome level. Short reads from Solexa/Illumina GA and ABI SOLiD platforms have been used to investigate the

SUBMITTER: Picardi E 

PROVIDER: S-EPMC2919710 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature