B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression.

ABSTRACT: Human tumors contain populations of both cancerous and host immune cells whose malignant signaling interactions may define each patient's disease trajectory. We used multiplexed phospho-flow cytometry to profile single cells within human follicular lymphoma tumors and discovered a subpopulation of lymphoma cells with impaired B cell antigen receptor (BCR) signaling. The abundance of BCR-insensitive cells in each tumor negatively correlated with overall patient survival. These lymphoma negative prognostic (LNP) cells increased as tumors relapsed following chemotherapy. Loss of antigen receptor expression did not explain the absence of BCR signaling in LNP tumor cells, and other signaling responses were intact in these cells. Furthermore, BCR signaling responses could be reactivated in LNP c

SUBMITTER: Irish JM 

PROVIDER: S-EPMC2919949 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature