Sequential events in the irreversible thermal denaturation of human brain-type creatine kinase by spectroscopic methods.

ABSTRACT: The non-cooperative or sequential events which occur during protein thermal denaturation are closely correlated with protein folding, stability, and physiological functions. In this research, the sequential events of human brain-type creatine kinase (hBBCK) thermal denaturation were studied by differential scanning calorimetry (DSC), CD, and intrinsic fluorescence spectroscopy. DSC experiments revealed that the thermal denaturation of hBBCK was calorimetrically irreversible. The existence of several endothermic peaks suggested that the denaturation involved stepwise conformational changes, which were further verified by the discrepancy in the transition curves obtained from various spectroscopic probes. During heating, the disruption of the active site structure occurred prior to the secon

SUBMITTER: Gao YS 

PROVIDER: S-EPMC2920553 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature