High-resolution characterization of Toxoplasma gondii transcriptome with a massive parallel sequencing method.

ABSTRACT: For the last couple of years, a method that permits the collection of precise positional information of transcriptional start sites (TSSs) together with digital information of the gene-expression levels in a high-throughput manner was established. We applied this novel method, 'tss-seq', to elucidate the transcriptome of tachyzoites of the Toxoplasma gondii, which resulted in the identification of 124,000 TSSs, and they were clustered into 10,000 transcription regions (TRs) with a statistics-based analysis. The TRs and annotated ORFs were paired, resulting in the identification of 30% of the TRs and 40% of the ORFs without their counterparts, which predicted undiscovered genes and stage-specific transcriptions, respectively. The massive data for TSSs make it possible to execute the first s

SUBMITTER: Yamagishi J 

PROVIDER: S-EPMC2920756 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature