Chromosomal instability in Streptomyces avermitilis: major deletion in the central region and stable circularized chromosome.

ABSTRACT:

Background

The chromosome of Streptomyces has been shown to be unstable, frequently undergoing gross chromosomal rearrangements. However, the mechanisms underlying this phenomenon remain unclear, with previous studies focused on two chromosomal ends as targets for rearrangements. Here we investigated chromosomal instability of Streptomyces avermitilis, an important producer of avermectins, and characterized four gross chromosomal rearrangement events, including a major deletion in the central region. The present findings provide a valuable contribution to the mechanistic study of genetic instability in Streptomyces.

Results

Thirty randomly-selected "bald" mutants derived from the wild-type strain all contained gross chromosomal rearrangements of various types. One of the bal

SUBMITTER: Chen W 

PROVIDER: S-EPMC2920896 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature