ATP-binding cassette transporter G1 deficiency dysregulates host defense in the lung.

ABSTRACT:

Rationale

Mice with genetic deletion of the cholesterol efflux transporter, ATP-binding cassette (ABC) G1, have pulmonary lipidosis and chronic pulmonary inflammation. Whether ABCG1 regulates host defense is unknown.

Objectives

To determine whether ABCG1 regulates pulmonary innate immunity and host defense, and to investigate the underlying molecular/cellular mechanisms.

Methods

Abcg1(+/+) and Abcg1(-/-) mice were challenged with intrapulmonary lipopolysaccharide (LPS) or Klebsiella pneumoniae, intravenous K. pneumoniae, or intraperitoneal LPS. Phenotypic responses were profiled. Bone marrow chimeras and in vitro assays were used to differentiate and characterize the role of hematopoietic versus nonhematopoietic ABCG1 in host defense.

Measurements and main results<

SUBMITTER: Draper DW 

PROVIDER: S-EPMC2921600 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature