Improved sensitivity of DNA microarrays using photonic crystal enhanced fluorescence.

ABSTRACT: DNA microarrays are used to profile changes in gene expression between samples in a high-throughput manner, but measurements of genes with low expression levels can be problematic with standard microarray substrates. In this work, we expand the detection capabilities of a standard microarray experiment using a photonic crystal (PC) surface that enhances fluorescence observed from microarray spots. This PC is inexpensively and uniformly fabricated using a nanoreplica molding technique, with very little variation in its optical properties within- and between-devices. By using standard protocols to process glass microarray substrates in parallel with PCs, we evaluated the impact of this substrate on a one-color microarray experiment comparing gene expression in two developmental stages of Gly

SUBMITTER: Mathias PC 

PROVIDER: S-EPMC2921648 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature