Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.

ABSTRACT: Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We developed methodologies for simultaneously quantifying blood flow (velocity, flux, hematocrit and shear rate) in extended networks at single-capillary resolution in vivo. Our approach relies on deconvolution of signals produced by labeled red blood cells as they move relative to the scanning laser of a confocal or multiphoton microscope and provides fully resolved three-dimensional flow profiles within vessel netwo

SUBMITTER: Kamoun WS 

PROVIDER: S-EPMC2921873 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature