New method for selection of hydrogen peroxide adapted bifidobacteria cells using continuous culture and immobilized cell technology.

ABSTRACT:

Background

Oxidative stress can severely compromise viability of bifidobacteria. Exposure of Bifidobacterium cells to oxygen causes accumulation of reactive oxygen species, mainly hydrogen peroxide, leading to cell death. In this study, we tested the suitability of continuous culture under increasing selective pressure combined with immobilized cell technology for the selection of hydrogen peroxide adapted Bifidobacterium cells. Cells of B. longum NCC2705 were immobilized in gellan-xanthan gum gel beads and used to continuously ferment MRS medium containing increasing concentration of H2O2 from 0 to 130 ppm.

Results

At the beginning of the culture, high cell density of 10(13) CFU per litre of reactor was tested. The continuous culture gradually adapted to increasing H2O2 con

SUBMITTER: Mozzetti V 

PROVIDER: S-EPMC2922086 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature