Spatiotemporal patterns of macrophage migration inhibitory factor (Mif) expression in the mouse placenta.

ABSTRACT:

Background

Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the immune response. The conspicuous expression of MIF during human implantation and early embryonic development also suggests this factor acts in reproductive functions. The overall goal of this study was to evaluate Mif expression by trophoblast and embryo placental cells during mouse pregnancy.

Methods

Mif was immunolocalized at implantation sites on gestation days (gd) 7.5, 10.5, 13.5 and 17.5. Ectoplacental cones and fetal placentas dissected from the maternal tissues were used for Western blotting and qRT-PCR assays on the same gestation days.

Results

During the

SUBMITTER: Faria MR 

PROVIDER: S-EPMC2922212 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature