Role of calpain-mediated p53 truncation in semaphorin 3A-induced axonal growth regulation.

ABSTRACT: Neurite outgrowth represents a critical stage in the correct development of neuronal circuitries, and is dependent on the complex regulation of actin filament and microtubule dynamics by intrinsic as well as extrinsic signals. Previous studies have implicated the tumor suppressor factor, p53, in the regulation of axonal outgrowth through a nontranscriptional effect involving local regulation of the Rho kinase signaling pathway that controls these dynamics. In the present study, we first showed that semaphorin 3A-induced growth cone collapse in cultured hippocampal neurons was associated with the partial truncation of phosphorylated p53, and that both effects were prevented by calpain inhibition with either m-calpain-specific siRNA or inhibitors. We further determined that semaphorin 3A-med

SUBMITTER: Qin Q 

PROVIDER: S-EPMC2922263 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature