Unknown

Dataset Information

0

NLR family member NLRC5 is a transcriptional regulator of MHC class I genes.


ABSTRACT: MHC class I plays a critical role in the immune defense against viruses and tumors by presenting antigens to CD8 T cells. An NLR protein, class II transactivator (CIITA), is a key regulator of MHC class II gene expression that associates and cooperates with transcription factors in the MHC class II promoter. Although CIITA also transactivates MHC class I gene promoters, loss of CIITA in humans and mice results in the severe reduction of only MHC class II expression, suggesting that additional mechanisms regulate the expression of MHC class I. Here, we identify another member of the NLR protein family, NLRC5, as a transcriptional regulator of MHC class I genes. Similar to CIITA, NLRC5 is an IFN-gamma-inducible nuclear protein, and the expression of NLRC5 resulted in enhanced MHC class I expression in lymphoid as well as epithelial cell lines. Using chromatin immunoprecipitation and reporter gene assays, we show that NLRC5 associates with and activates the promoters of MHC class I genes. Furthermore, we show that the IFN-gamma-induced up-regulation of MHC class I requires NLRC5, because knockdown of NLRC5 specifically impaired the expression of MHC class I. In addition to MHC class I genes, NLRC5 also induced the expression of beta2-microglobulin, transporter associated with antigen processing, and large multifunctional protease, which are essential for MHC class I antigen presentation. Our results suggest that NLRC5 is a transcriptional regulator, orchestrating the concerted expression of critical components in the MHC class I pathway.

SUBMITTER: Meissner TB 

PROVIDER: S-EPMC2922274 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

NLR family member NLRC5 is a transcriptional regulator of MHC class I genes.

Meissner Torsten B TB   Li Amy A   Biswas Amlan A   Lee Kyoung-Hee KH   Liu Yuen-Joyce YJ   Bayir Erkan E   Iliopoulos Dimitrios D   van den Elsen Peter J PJ   Kobayashi Koichi S KS  

Proceedings of the National Academy of Sciences of the United States of America 20100716 31


MHC class I plays a critical role in the immune defense against viruses and tumors by presenting antigens to CD8 T cells. An NLR protein, class II transactivator (CIITA), is a key regulator of MHC class II gene expression that associates and cooperates with transcription factors in the MHC class II promoter. Although CIITA also transactivates MHC class I gene promoters, loss of CIITA in humans and mice results in the severe reduction of only MHC class II expression, suggesting that additional me  ...[more]

Similar Datasets

2010-07-30 | E-GEOD-22064 | biostudies-arrayexpress
2010-06-30 | GSE22064 | GEO
| S-EPMC4374748 | biostudies-literature
2015-02-27 | E-GEOD-59092 | biostudies-arrayexpress
| S-EPMC3578426 | biostudies-literature
2015-02-27 | GSE59092 | GEO
| S-EPMC3346158 | biostudies-literature
| S-EPMC3345046 | biostudies-literature
2024-02-22 | GSE233194 | GEO
| S-EPMC3249515 | biostudies-literature