The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli.

ABSTRACT: Mobile genetic elements are widespread in bacteria, where they cause several kinds of mutations. Although their effects are on the whole negative, rare beneficial mutations caused by insertion sequence elements are frequently selected in some experimental evolution systems. For example, in earlier work, we found that strains of Escherichia coli that lack the sigma factor RpoS adapt to a high-osmolarity environment by the insertion of element IS10 into the promoter of the otsBA operon, rewiring expression from RpoS dependent to RpoS independent. We wished to determine how the presence of IS10 in the genome of this strain shaped the evolutionary outcome. IS10 could influence the outcome by causing mutations that confer adaptive phenotypes that cannot be achieved by strains without the elemen

SUBMITTER: Stoebel DM 

PROVIDER: S-EPMC2922620 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature