Prophossi: automating expert validation of phosphopeptide-spectrum matches from tandem mass spectrometry.

ABSTRACT:

Motivation

Complex patterns of protein phosphorylation mediate many cellular processes. Tandem mass spectrometry (MS/MS) is a powerful tool for identifying these post-translational modifications. In high-throughput experiments, mass spectrometry database search engines, such as MASCOT provide a ranked list of peptide identifications based on hundreds of thousands of MS/MS spectra obtained in a mass spectrometry experiment. These search results are not in themselves sufficient for confident assignment of phosphorylation sites as identification of characteristic mass differences requires time-consuming manual assessment of the spectra by an experienced analyst. The time required for manual assessment has previously rendered high-throughput confident assignment of phosphorylation site

SUBMITTER: Martin DM 

PROVIDER: S-EPMC2922888 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature