Genetic integrity of the human Y chromosome exposed to groundwater arsenic.

ABSTRACT:

Background

Arsenic is a known human carcinogen reported to cause chromosomal deletions and genetic anomalies in cultured cells. The vast human population inhabiting the Ganges delta in West Bengal, India and Bangladesh is exposed to critical levels of arsenic present in the groundwater. The genetic and physiological mechanism of arsenic toxicity in the human body is yet to be fully established. In addition, lack of animal models has made work on this line even more challenging.

Methods

Human male blood samples were collected with their informed consent from 5 districts in West Bengal having groundwater arsenic level more than 50 microg/L. Isolation of genomic DNA and preparation of metaphase chromosomes was done using standard protocols. End point PCR was performed for estab

SUBMITTER: Ali S 

PROVIDER: S-EPMC2923088 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature