Retrotransposon vectors for gene delivery in plants.

ABSTRACT:

Background

Retrotransposons are abundant components of plant genomes, and although some plant retrotransposons have been used as insertional mutagens, these mobile genetic elements have not been widely exploited for plant genome manipulation. In vertebrates and yeast, retrotransposons and retroviruses are routinely altered to carry additional genes that are copied into complementary (c)DNA through reverse transcription. Integration of cDNA results in gene delivery; recombination of cDNA with homologous chromosomal sequences can create targeted gene modifications. Plant retrotransposon-based vectors, therefore, may provide new opportunities for plant genome engineering.

Results

A retrotransposon vector system was developed for gene delivery in plants based on the Tnt1 element

SUBMITTER: Hou Y 

PROVIDER: S-EPMC2923131 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature