Rhabdastrellic acid-A induced autophagy-associated cell death through blocking Akt pathway in human cancer cells.

ABSTRACT:

Background

Autophagy is an evolutionarily conserved protein degradation pathway. A defect in autophagy may contribute to tumorigenesis. Autophagy inducers could have a potential function in tumor prevention and treatment.

Methodology/principal findings

Our results showed that Rhabdastrellic acid-A, an isomalabaricane triterpenoid isolated from the sponge Rhabdastrella globostellata, inhibited proliferation of human cancer cell lines Hep3B and A549 and induced caspase-independent cell death in both the cell lines. Further investigation showed that Rhabdastrellic acid-A induced autophagy of cancer cells determined by YFP-LC3 punctation and increased LC3-II. The pretreatment with autophagy inhibitor 3-MA inhibited Rhabdastrellic acid-A-induced cell death. Knockdown of autophagy

SUBMITTER: Li DD 

PROVIDER: S-EPMC2923153 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature