Defining reference genes in Oryza sativa using organ, development, biotic and abiotic transcriptome datasets.

ABSTRACT:

Background

Reference genes are widely used to normalise transcript abundance data determined by quantitative RT-PCR and microarrays. However, the approaches taken to define reference genes can be variable. Although Oryza sativa (rice) is a widely used model plant and important crop specie, there has been no comprehensive analysis carried out to define superior reference genes.

Results

Analysis of 136 Affymetrix transcriptome datasets comprising of 373 genome microarrays from studies in rice that encompass tissue, developmental, abiotic, biotic and hormonal transcriptome datasets identified 151 genes whose expression was considered relatively stable under all conditions. A sub-set of 12 of these genes were validated by quantitative RT-PCR and were seen to be stable under a nu

SUBMITTER: Narsai R 

PROVIDER: S-EPMC2923530 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature