Tissue-specific and ubiquitous expression patterns from alternative promoters of human genes.

ABSTRACT:

Background

Transcriptome diversity provides the key to cellular identity. One important contribution to expression diversity is the use of alternative promoters, which creates mRNA isoforms by expanding the choice of transcription initiation sites of a gene. The proximity of the basal promoter to the transcription initiation site enables prediction of a promoter's location based on the gene annotations. We show that annotation of alternative promoters regulating expression of transcripts with distinct first exons enables a novel methodology to quantify expression levels and tissue specificity of mRNA isoforms.

Principal findings

The use of distinct alternative first exons in 3,296 genes was examined using exon-microarray data from 11 human tissues. Comparing two transcripts

SUBMITTER: Jacox E 

PROVIDER: S-EPMC2923625 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature