Environmental and genetic factors that contribute to Escherichia coli K-12 biofilm formation.

ABSTRACT: Biofilms are communities of bacteria whose formation on surfaces requires a large portion of the bacteria's transcriptional network. To identify environmental conditions and transcriptional regulators that contribute to sensing these conditions, we used a high-throughput approach to monitor biofilm biomass produced by an isogenic set of Escherichia coli K-12 strains grown under combinations of environmental conditions. Of the environmental combinations, growth in tryptic soy broth at 37 degrees C supported the most biofilm production. To analyze the complex relationships between the diverse cell-surface organelles, transcriptional regulators, and metabolic enzymes represented by the tested mutant set, we used a novel vector-item pattern-mining algorithm. The algorithm related biofilm amoun

SUBMITTER: Pruss BM 

PROVIDER: S-EPMC2923660 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature